Sharanappa Achappa
,
S. V. Desai
*
- Department of Biotechnology, KLE Technological University, Hubballi - 580 031, India
Abstract
The central dogma of life beginning with DNA leading to RNA and subsequently to protein is the unifying principle and corner stone to the understanding of biology. With the advent of sophisticated and high throughput sequencing, enormous data is being generated. This has made the bio-informatics tools indispensable to decipher the data into meaningful conclusions. The objective of the present study was to comprehend the understanding of Genomics and Proteomics with the use of Bioinformatics tools. The course project activity was performed for Genomics & Proteomics course of VII semester undergraduate students of Engineering in Biotechnology. The students were grouped into team of four and assigned with one pathogenic bacterium. The activity involved identifying a pathogenesis-related protein, retrieving the DNA sequences of the protein, finding conserved domains, building phylogenetic relationship amongst the homologous organisms, genome annotation, predicting secondary and tertiary structure of proteins and deducing its stability. In the process, the students were engaged in active learning by the use of various Bioinformatics tools and repositories. The students were thus able to predict the secondary and tertiary structure of protein beginning with DNA sequences. The activity was instrumental in addressing the Graduate attributes. Rubrics-based assessment was performed to measure the attainment level on the scale of 10. The attainment ranged from 7.28 to 9.25 for different performance indicator (PI) of the graduate attributes. A formal anonymous feedback from the students revealed that the activity helped in appreciating the intricate relationship between Genomics and Proteomics by integrating the related concepts.
References
- Yun-Can Ai, �Teaching bioinformatics: A student-centred and problem-based approach,� The China Papers' Issue 1, October 2002, (2002).
- Bednarski, A.E., Elgin., S.C.R., Pakrasi, H.B.(2005). An Inquiry into Protein Structure and Genetic Disease: Introducing Undergraduates to Bioinformatics in a Large Introductory Course. Cell Biology Education.4(3), 207-220.
- Ostrowski, J., Lucjan S Wyrwicz, L.S. (2009). Integrating genomics, proteomics and bioinformatics in translational studies of molecular medicine.Expert Review in Molecular Diagnostics. 9 (6), 623-630.
- Rastogi,S.C., Rastogi, P., Namita Mendiratta. (2008). Bioinformatics Methods And Applications: GenomicsProteomics And Drug Discovery, 3rd Edition. PHI Learning Pvt. Ltd.New Delhi.
- Berrar D., Granzow M., Dubitzky W. (2007) Introduction to Genomic and Proteomic Data Analysis. In: Dubitzky W., Granzow M., Berrar D. (eds) Fundamentals of Data Mining in Genomics and Proteomics. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-47509-7_1
- Horgan RP, Kenny LC. �Omic� technologies: genomics, transcriptomics, proteomics and metabolomics. The Obstetrician & Gynaecologist 2011; 13:189�195
- Claudia Manzoni, Demis A Kia, Jana Vandrovcova, John Hardy, Nicholas W Wood, Patrick A Lewis, Raffaele Ferrari, Genome, transcriptome and proteome: the rise of omics data and their integration in biomedical sciences, Briefings in Bioinformatics, Volume 19, Issue 2, March 2018, Pages 286�302, https://doi.org/10.1093/bib/bbw114.
- W. Setiawan, "Implementation of Internet of Things in Biomedical Measurement and Instrumentation Course Project," 2020 IEEE Global Engineering Education Conference (EDUCON), Porto, Portugal, 2020, pp. 1657 1661, doi: 10.1109/EDUCON45650.2020.9125197.
- Guidelines for the implementation of the course project on the discipline "Human resource management�: for students of specialty 073 "Management"/comp. T. Pohorielova; National Technical University "Kharkiv Polytechnic Institute". � Kharkiv : Modelist, 2020. � 22 p.
- Sharanappa Achappa, L. R. Patil, V. S. Hombalimath, Anil R. Shet (2020); DOI: 10.16920/jeet/2020/v33i0/150154
- Sharanappa Achappa, L. R. Patil, V. S. Hombalimath, Anil R. Shet (2018); DOI: 10.16920/jeet/2018/v0i0/120904.
